O-012. Intracytoplasmic sperm injection into immature human oocytes

Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection.

OBJECTIVE To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure. DESIGN Comparison of the rate of DNA fragmentation in human oocytes at different stages of maturation soon after pick-up (control) and in unfertilized oocytes after ICSI...

Fertilization and pregnancy potential of immature oocytes from stimulated intracytoplasmic sperm injection cycles

OBJECTIVE We evaluated the fertilization potential of immature oocytes obtained from controlled ovarian hyperstimulation cycles of patients undergoing ICSI. METHODS We retrospectively analyzed 463 ICSI cycles containing at least one immature oocyte at oocyte denudation. ICSI was performed on mature oocytes at oocyte denudation (metaphase-II [MII] oocytes) and the oocytes that extruded the fir...

Pregnancy and fertilization potential of immature oocytes retrieved in intracytoplasmic sperm injection cycles

OBJECTIVE The goal of this study was to evaluate the pregnancy potential of immature (metaphase I or germinal vesicle stage) oocytes retrieved in intracytoplasmic sperm injection (ICSI) cycles. METHODS A total of 1,871 couples with infertility underwent 2,984 ICSI cycles. Cycles in which three or fewer oocytes were retrieved were included in this study in order to evaluate the pregnancy poten...

Human Sperm Aster Formation and Chromatin Configuration in Rabbit Oocytes Following Intracytoplasmic Sperm Injection Using a Piezo-Micromanipulator

In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

Intracytoplasmic sperm injection (ICSI).